Apelin enhances IL-1β expression in human synovial fibroblasts by inhibiting miR-144-3p through the PI3K and ERK pathways
Ting‐Kuo Chang, Yuhan Wang, Shu‐Jui Kuo, Shih‐Wei Wang, Chun‐Hao Tsai, Yi‐Chin Fong, Nan‐Lin Wu, Shan‐Chi Liu, Chih‐Hsin Tang
Abstract
. Our evidence is the first to show that APLN stimulates the expression of IL-1β by activating the PI3K and ERK pathway and suppressing downstream expression of miRNA-144-3p in OASFs. We also demonstrate that knockdown of APLN expression by shAPLN transfection ameliorated changes in OA cartilage severity. These results shed light on OA pathogenesis and suggest a novel treatment pathway.
Topics & Concepts
ApelinPI3K/AKT/mTOR pathwayMAPK/ERK pathwayMedicineAdipokineGene knockdownProinflammatory cytokineSynovial jointCancer researchOsteoarthritisCartilageProtein kinase BSynovial fluidmicroRNASignal transductionInternal medicineCell biologyInflammationPathologyBiologyLeptinApoptosisAnatomyReceptorAlternative medicineGeneArticular cartilageObesityBiochemistryApelin-related biomedical researchCardiovascular, Neuropeptides, and Oxidative Stress ResearchLipid metabolism and disorders