Rapid and label-free detection of aflatoxin B1 using a rationally truncated aptamer and <i>via</i> circular dichroism measurement
Chao Wang, Kexiao Zhu, Pengfei Shi, Xilai Ding, Shusheng Zhang
Abstract
We describe a simple aptamer assay for the rapid analysis of aflatoxin B1 (AFB1), a potently carcinogenic fungal metabolite. The original anti-AFB1 aptamer was rationally truncated, obtaining a short aptamer that displayed better performance. Different concentrations of AFB1 were detected by measuring the circular dichroism spectra of this short aptamer. The detection limit reached 0.6 nM, which was lower than that of the original aptamer (80 nM).
Topics & Concepts
AptamerCircular dichroismAflatoxinDetection limitChemistryChromatographyAnalytical Chemistry (journal)StereochemistryMolecular biologyBiologyFood scienceAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionDNA and Nucleic Acid Chemistry