Targeted DNA methylation analysis and prediction of smoking habits in blood based on massively parallel sequencing
Athina Vidaki, Benjamin Planterose Jiménez, Brando Poggiali, Vivian Kalamara, Kristiaan J. van der Gaag, Silvana C. E. Maas, Mohsen Ghanbari, Titia Sijen, Manfred Kayser
Abstract
-score > 0.8; 2-categories). Overall, our novel assay takes us one step closer towards the forensic application of viable smoking habit prediction from blood traces. However, future research is needed towards forensically validating the assay, especially in terms of sensitivity. We also need to further shed light on the employed biomarkers, particularly on the mechanistics, tissue specificity and putative confounders of smoking epigenetic signatures.
Topics & Concepts
DNA methylationMethylationCpG siteContext (archaeology)Bisulfite sequencingMultiplexMedicineMassive parallel sequencingBiologyOncologyBioinformaticsGeneticsComputational biologyDNA sequencingDNAGeneGene expressionPaleontologyEpigenetics and DNA MethylationCancer-related molecular mechanisms researchRNA modifications and cancer