Enhancing the thermostability of phospholipase D from Streptomyces halstedii by directed evolution and elucidating the mechanism of a key amino acid residue using molecular dynamics simulation
Lin Huang, Jieying Ma, Jingcheng Sang, Nan Wang, Shuang Wang, Chen Wang, Hongwei Kang, Fufeng Liu, Fuping Lu, Yihan Liu
Topics & Concepts
ThermostabilityMutantSalt bridgeMolecular dynamicsChemistryPhospholipase DWild typeProtein engineeringBiochemistryStereochemistryBiophysicsBiologyEnzymeComputational chemistryGeneEnzyme Structure and FunctionProtein Structure and DynamicsProtein Kinase Regulation and GTPase Signaling