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Enhancing the thermostability of phospholipase D from Streptomyces halstedii by directed evolution and elucidating the mechanism of a key amino acid residue using molecular dynamics simulation

Lin Huang, Jieying Ma, Jingcheng Sang, Nan Wang, Shuang Wang, Chen Wang, Hongwei Kang, Fufeng Liu, Fuping Lu, Yihan Liu

2020International Journal of Biological Macromolecules27 citationsDOI

Topics & Concepts

ThermostabilityMutantSalt bridgeMolecular dynamicsChemistryPhospholipase DWild typeProtein engineeringBiochemistryStereochemistryBiophysicsBiologyEnzymeComputational chemistryGeneEnzyme Structure and FunctionProtein Structure and DynamicsProtein Kinase Regulation and GTPase Signaling
Enhancing the thermostability of phospholipase D from Streptomyces halstedii by directed evolution and elucidating the mechanism of a key amino acid residue using molecular dynamics simulation | Litcius