Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

Abstract

Abstract Background Eimeria stiedae parasitizes the bile duct, causing hepatic coccidiosis in rabbits. Coccidiosis control using anticoccidials led to drug resistance and residues; therefore, vaccines are required as an alternative control strategy. Apical membrane antigen 1 (AMA1) and immune mapped protein 1 (IMP1) are surface-located proteins that might contribute to host cell invasion, having potential as candidate vaccine antigens. Methods Herein, we cloned and expressed the E. stiedae Es AMA1 and Es IMP1 genes. The reactogenicity of recombinant AMA1 (r Es AMA1) and IMP1 (r Es IMP1) proteins were investigated using immunoblotting. For the vaccination-infection trial, rabbits were vaccinated with r Es AMA1 and r Es IMP1 (both 100 μg/rabbit) twice at 2-week intervals. After vaccination, various serum cytokines were measured. The protective effects of r Es AMA1 and r Es IMP1 against E. stiedae infection were assessed using several indicators. Sera were collected weekly to detect the specific antibody levels. Results Both r Es AMA1 and r Es IMP1 showed strong reactogenicity. Rabbits vaccinated with r Es AMA1 and r Es IMP1 displayed significantly increased serum IL-2 ( F (4, 25) = 9.53, P = 0.000), IL-4 ( F (4, 25) = 7.81, P = 0.000), IL-17 ( F (4, 25) = 8.55, P = 0.000), and IFN-γ ( F (4, 25) = 6.89, P = 0.001) levels; in the r Es IMP1 group, serum TGF-β1 level was also elevated ( F (4, 25) = 3.01, P = 0.037). After vaccination, the specific antibody levels increased and were maintained at a high level. The vaccination-infection trial showed that compared with the positive control groups, rabbits vaccinated with the recombinant proteins showed significantly reduced oocyst output ( F (5, 54) = 187.87, P = 0.000), liver index ( F (5, 54) = 37.52, P = 0.000), and feed conversion ratio; body weight gain was significantly improved ( F (5, 54) = 28.82, P = 0.000). Conclusions r Es AMA1 and r Es IMP1 could induce cellular and humoral immunity, protecting against E. stiedae infection. Thus, r Es AMA1 and r Es IMP1 are potential vaccine candidates against E. stiedae . Graphic abstract

Topics & Concepts