<i>In Vitro</i>Exposure of Leukocytes to HIV Preexposure Prophylaxis Decreases Mitochondrial Function and Alters Gene Expression Profiles
Emily Bowman, Cheryl Cameron, Brian Richardson, Manjusha Kulkarni, Janelle Gabriel, Aaren Kettelhut, Lane Hornsby, Jesse J. Kwiek, Abigail Norris Turner, Carlos Malvestutto, José A. Bazan, Susan L. Koletar, Susanne Doblecki‐Lewis, Michael M. Lederman, Mark J. Cameron, Nichole R. Klatt, Jordan E. Lake, Nicholas Funderburg
Abstract
The use of antiretroviral therapy (ART) as preexposure prophylaxis (PrEP) is an effective strategy for preventing HIV acquisition. The cellular consequences of PrEP exposure, however, have not been sufficiently explored to determine potential effects on health in individuals without HIV. In this study, peripheral blood mononuclear cells (PBMCs) from people without HIV were exposed to tenofovir disoproxil fumarate (TDF) or emtricitabine (FTC) overnight. Mitochondrial mass and function were measured by flow cytometry and an Agilent XFp analyzer.