The Heterotrophic Bacterium Cupriavidus pinatubonensis JMP134 Oxidizes Sulfide to Sulfate with Thiosulfate as a Key Intermediate
Yufeng Xin, Rui Gao, Feifei Cui, Chuanjuan Lü, Honglei Liu, Huaiwei Liu, Yongzhen Xia, Luying Xun
Abstract
We establish a new pathway of sulfide oxidation with thiosulfate as a key intermediate in Cupriavidus pinatubonensis JMP134. The bacterium mainly oxidizes sulfide by using sulfide:quinone oxidoreductase, persulfide dioxygenase, and the Sox system with thiosulfate as a key intermediate. Although the purified and reconstituted Sox system oxidizes sulfide, its rate of sulfide oxidation in C. pinatubonensis JMP134 is too low to be physiologically relevant. The findings reveal how these sulfur-oxidizing enzymes participate in sulfide oxidation in a single bacterium.
Topics & Concepts
ThiosulfateSulfideSulfurChemistryOxidizing agentBacteriaDioxygenaseSulfate-reducing bacteriaCombinatorial chemistryBiochemistrySulfateEnzymeOrganic chemistryBiologyGeneticsMetal Extraction and BioleachingArsenic contamination and mitigationChromium effects and bioremediation