Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade
Zhenyang Wang, Wenbin Liu, Wei Liu, Yuanyuan Ma, Yatong Li, Baoqi Wang, Xiaozhen Wei, Zhiming Liu, Hao Song
Abstract
. Eventually, after 4 and 8 reused cycles, the co-immobilized enzymes retained 72.5% and 53.1% of their original activity, respectively, showing a high stability to minimize the total cost of enzymes and suggesting that the co-immobilized UGTs is of potentially signficant value for the production of Reb M.
Topics & Concepts
Rebaudioside ARecombinant DNACascadeGlycosyltransferaseChemistryCombinatorial chemistryBiochemistryChromatographyEnzymeSteviosideMedicineGenePathologyAlternative medicineCarbohydrate Chemistry and SynthesisEnzyme Catalysis and ImmobilizationGlycosylation and Glycoproteins Research