Semi-rationally designed site-saturation mutation of Helicobacter pylori α-1,2-fucosyltransferase for improved catalytic activity and thermostability
Mengli Li, Tao Zhang, Chenchen Li, Wei Gao, Zhu Liu, Ming Miao
Topics & Concepts
ThermostabilityEnzyme kineticsMutantChemistryDirected evolutionActive siteEnzymeEnzyme assaySaturated mutagenesisBiochemistryGeneGlycosylation and Glycoproteins ResearchEnzyme Production and CharacterizationCarbohydrate Chemistry and Synthesis