Overlapping variants in the blood, tissues and cell lines for patients with intracranial meningiomas are predominant in stem cell-related genes
Deema Hussein, Ashraf Dallol, Rita Quintas‐Rey, Hans-Juergen Schulten, Mona Alomari, Saleh Baeesa, Mohammed Bangash, Fahad Alghamdi, Ishaq Khan, M‐Zaki ElAssouli, Mohamad Saka, Ãngel Carracedo, Adeel Chaudhary, Adel Abuzenadah
Abstract
OBJECTIVE: Bulk tissue genomic analysis of meningiomas identified common somatic mutations, however, it often excluded blood-related variants. In contrast, genomic characterisation of primary cell lines that can provide critical information regarding growth and proliferation, have been rare. In our work, we identified the variants that are present in the blood, tissues and corresponding cell lines that are likely to be predictive, tumorigenic and progressive. METHOD: Whole-exome sequencing was used to identify variants and distinguish related pathways that exist in 42 blood, tissues and corresponding cell lines (BTCs) samples for patients with intracranial meningiomas. Conventional sequencing was used for the confirmation of variants. Integrative analysis of the gene expression for the corresponding samples was utilised for further interpretations. RESULTS: gene CONCLUSION: Stem cell-related pathways and genes showed high prevalence in the BTC variants, and novel variants in stem cell-related genes were identified for meningioma. These variants can potentially be used as predictive, tumorigenic and progressive biomarkers for meningioma.