c-di-AMP Accumulation Impairs Muropeptide Synthesis in Listeria monocytogenes
Steven Massa, Amar Deep Sharma, Cheta Siletti, Zepeng Tu, Jared J. Godfrey, William G. Gutheil, TuAnh N. Huynh
Abstract
The bacterial second messenger c-di-AMP is produced by a large number of bacteria and conditionally essential to many species. Conversely, c-di-AMP accumulation is also toxic to bacterial physiology and pathogenesis, but its mechanisms are largely undefined. We found that in Listeria monocytogenes , elevated c-di-AMP levels diminished muropeptide synthesis and increased susceptibility to cell wall-targeting antimicrobials. Cell wall defects might be an important mechanism for attenuated virulence in bacteria with high c-di-AMP levels.
Topics & Concepts
Listeria monocytogenesBiologyBacteriaMicrobiologyVirulenceCell wallListeriaBacterial cell structurePathogenesisCellBiochemistryImmunologyGeneGeneticsListeria monocytogenes in Food SafetyProbiotics and Fermented FoodsBacterial biofilms and quorum sensing