Development of a Broth Microdilution Method To Characterize Chlorhexidine MICs among Bacteria Collected from 2005 to 2019 at Three U.S. Sites
Joseph D. Lutgring, Julian E. Grass, David Lonsway, Byong Kwon Yoo, Erin Epson, Megan Crumpler, Karen Galliher, Kathleen O’Donnell, Matthew Zahn, Eric Evans, Jesse T. Jacob, Alexander M. Page, Sarah W. Satola, Gillian Smith, Marion Kainer, Daniel Muleta, Christopher Wilson, Mary K. Hayden, Sujan Reddy, Christopher A. Elkins, J. Kamile Rasheed, Maria Karlsson, Shelley S. Magill, Alice Guh
Abstract
Chlorhexidine bathing to prevent transmission of multidrug-resistant organisms and reduce health care-associated infections has been adopted by many hospitals. There is concern about the possible unintended consequences of using this agent widely. One possible unintended consequence is decreased susceptibility to chlorhexidine, but there are not readily available methods to perform this evaluation. We developed a method for chlorhexidine MIC testing that can be used to evaluate for possible unintended consequences.