Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid
Can Zhang, Xiaoxiao Wu, Dongyang Li, Jinnuo Hu, Debin Wan, Zhen Zhang, Bruce D. Hammock
Abstract
, respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA.
Topics & Concepts
ImmunoassayColloidal goldFlow (mathematics)NanoparticleChemistryChromatographyAnalytical Chemistry (journal)NanotechnologyMaterials scienceAntibodyPhysicsImmunologyBiologyMechanicsBiosensors and Analytical DetectionAdvanced Biosensing Techniques and ApplicationsMonoclonal and Polyclonal Antibodies Research