A sensitive fluorescent probe for β-galactosidase activity detection and application in ovarian tumor imaging
Fan Fang, Li Zhang, Xinguang Zhou, Fang-Ya Mu, Guoyue Shi
Abstract
. We used DCMCA-β-gal to detect and visualize β-gal activity in SKOV3 human ovarian cancer cells, as well as for real-time imaging of β-gal activity in ovarian cancer mouse models. DCMCA-β-gal possessed high sensitivity, "turn-on" NIR emission, a large spectral shift, and high photostability in a dynamic living system and thus could serve as a highly sensitive sensor for real-time tracking of β-gal activity in vivo and ovarian tumor imaging.
Topics & Concepts
Ovarian cancerIn vivoFluorescenceOvarian tumorCancer researchDetection limitBiomarkerFluorescence-lifetime imaging microscopyCancerMaterials scienceChemistryBiologyMedicineBiochemistryInternal medicineOpticsChromatographyPhysicsBiotechnologyNanoplatforms for cancer theranosticsAdvanced biosensing and bioanalysis techniquesNanoparticle-Based Drug Delivery