In vivo [U-<sup>13</sup>C]glucose labeling to assess heart metabolism in murine models of pressure and volume overload
Moritz Schnelle, Mei Chong, Anna Zoccarato, Manar Elkenani, Greta J. Sawyer, Gerd Hasenfuß, Christian Ludwig, Ajay M. Shah
Abstract
This study implemented a method for assessing the fate of glucose carbons in the heart in vivo and used this to demonstrate that pressure and volume overload are associated with distinct changes. In contrast to volume overload, pressure overload-induced changes affect the tricarboxylic acid cycle, glycolytic pathways, and glutamine synthesis. A better understanding of cardiac glucose metabolism under pathological conditions in vivo may provide new therapeutic strategies specific for different types of hemodynamic overload.
Topics & Concepts
In vivoVolume (thermodynamics)Volume overloadPressure overloadCarbohydrate metabolismChemistryInternal medicineCardiologyMedicineBiologyPhysicsHeart failureBiochemistryThermodynamicsCardiac hypertrophyBiotechnologyCardiovascular Function and Risk FactorsDiet and metabolism studiesAdipose Tissue and Metabolism