Effective model of loop extrusion predicts chromosomal domains

Abstract

An active loop-extrusion mechanism is regarded as the main out-of-equilibrium mechanism responsible for the structuring of megabase-sized domains in chromosomes. We developed a model to study the dynamics of the chromosome fiber by solving the kinetic equations associated with the motion of the extruder. By averaging out the position of the extruder along the chain, we build an effective equilibrium model capable of reproducing experimental contact maps based solely on the positions of extrusion-blocking proteins. We assessed the quality of the effective model using numerical simulations of chromosomal segments and comparing the results with explicit-extruder models and experimental data.

Topics & Concepts