<i>In vivo</i>monitoring of tissue regeneration using a ratiometric lysosomal AIE probe
Xiujuan Shi, Neng Yan, Guangle Niu, Simon H. P. Sung, Zhiyang Liu, Junkai Liu, Ryan T. K. Kwok, Jacky W. Y. Lam, Wen‐Xiong Wang, Herman H. Y. Sung, Ian D. Williams, Ben Zhong Tang
Abstract
, good biocompatibility and excellent photostability. The caudal fin regeneration of a fish model (medaka larvae) was monitored by tracking the lysosomal pH change. It was found that the mean lysosomal pH is reduced during 24-48 hpa to promote the autophagic activity for cell debris degradation. Our research can quantify the changes in mean lysosomal pH and also exhibit its distribution during the caudal fin regeneration. We believe that the AIE-active lysosomal pH probe can also be potentially used for long-term tracking of various lysosome-involved biological processes, such as tracking the stress responses of tissue, tracking the inflammatory responses, and so on.