Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting
Yancen Dai, Yuko Sato, Bo Zhu, Tetsuya Kitaguchi, Hiroshi Kimurâ, Farid J. Ghadessy, Hiroshi Ueda
Abstract
with a limit of detection of 0.72 nM. In fixed and live cells, 8.3- and 8.4-fold enhancement was respectively observed. Furthermore, we demonstrate live-cell sorting based on p53 expression. This study provides the first evidence of the feasibility and applicability of Q-body probes for the live-cell imaging of intrinsically intracellular proteins and opens a novel avenue for research and diagnostic applications on intracellular target-based live-cell sorting.
Topics & Concepts
IntracellularCell sortingCell biologyLive cell imagingMonoclonal antibodyCellChemistryAntibodyFluorescenceSortingFluorescence-lifetime imaging microscopyBiophysicsBiologyBiochemistryImmunologyComputer scienceProgramming languageQuantum mechanicsPhysicsMonoclonal and Polyclonal Antibodies ResearchClick Chemistry and ApplicationsAdvanced biosensing and bioanalysis techniques