The enhancement of enzyme cascading <i>via</i> tetrahedral DNA framework modification
Haipei Zhao, Mingqiang Li, Mingqiang Li, Shasha Lu, Nan Cao, Xiaolei Zuo, Shaopeng Wang, Min Li, Min Li
Abstract
is enzyme modification. However, modifying enzymes without sacrificing their activity remains challenging until now. For example, labeling enzymes with DNA, one of the well-established enzyme modification methods, has been shown to significantly reduce the enzymatic activity. Herein we report an enzyme conjugation method that can rescue the reduction of enzymatic activity caused by DNA labeling. We demonstrate that immobilizing DNA-modified enzymes on the vertex of TDNs (tetrahedral DNA nanostructures) enhances the enzymatic activity compared with their unmodified counterparts. Using this strategy, we have further developed an ultra-sensitive and high-throughput electrochemical biosensor for sarcosine detection, which holds great promise for prostate cancer screening.