Microsphere mediated exosome isolation and ultra-sensitive detection on a dielectrophoresis integrated microfluidic device
Wenjie Zhao, Lingqian Zhang, Yifei Ye, Yuang Li, Xiaofeng Luan, Jinlong Liu, Jie Cheng, Yang Zhao, Mingxiao Li, Chengjun Huang
Abstract
exosomes per mL with a detection limit of 193 exosomes per mL was obtained. Through monitoring five proteins (CD81, CEA, EpCAM, CD147, and AFP) of exosomes from three different cell lines (A549, HEK293, and HepG2), a significant difference in marker expression levels was observed in different cell lines. Therefore, this method has good prospects in exosome-based tumor marker detection and cancer diagnosis.
Topics & Concepts
MicrosphereDielectrophoresisMicrofluidicsDetection limitExosomeMaterials scienceIsolation (microbiology)NanotechnologyMicrofluidic chipFluorescenceLab-on-a-chipMicrovesiclesChemistryChromatographyBiologyOpticsBioinformaticsEngineeringmicroRNAPhysicsGeneChemical engineeringBiochemistryExtracellular vesicles in diseaseMicrofluidic and Bio-sensing TechnologiesAnodic Oxide Films and Nanostructures