Establishment of a human induced pluripotent stem cell (iPSC) line (JUCTCi010-A) from a healthy Jordanian female skin dermal fibroblasts
Nidaa A. Ababneh, Ban Al-Kurdi, Dema Ali, Duaa Abuarqoub, Raghda Barham, Bareqa Salah, Abdalla Awidi
Abstract
Human integration-free induced pluripotent pluripotent stem cells (hiPSCs) were generated from skin fibroblasts obtained from a 27-year-old healthy Jordanian female. The resulting iPSCs expressed the most common pluripotency stem cell markers, they retained the normal karyotype similar to the original fibroblasts and showed the potential to differentiate into three germ layers in vitro. This iPSC line could serve as a wild-type control that can be used in hereditary disease modeling studies and in optimization of different differentiation protocols.
Topics & Concepts
Induced pluripotent stem cellBiologyGerm layerKaryotypeCell cultureCell biologyStem cellHuman Induced Pluripotent Stem CellsIn vitroEmbryonic stem cellGeneticsChromosomeGenePluripotent Stem Cells ResearchCRISPR and Genetic EngineeringBiomedical Ethics and Regulation