Establishment and characterization of two iPSC lines derived from healthy controls
Achchhe Patel, Alejandro Garcia Diaz, Jennifer C. Moore, Dario Sirabella, Barbara Corneo
Abstract
We have generated two iPSC lines from skin biopsies of two healthy individuals. Skin fibroblasts were derived and reprogrammed using a Sendai virus-based approach. The resulting iPSC lines have normal karyotype, express stemness markers and can generate endoderm, mesoderm and ectoderm in vitro. These iPSC lines can be used as healthy controls in differentiation paradigms as well as backbone for gene editing experiments.
Topics & Concepts
BiologyEndodermMesodermEctodermKaryotypeSendai virusGerm layerInduced pluripotent stem cellIn vitroCell biologyGeneticsEmbryoGeneCellular differentiationEmbryonic stem cellEmbryogenesisChromosomeCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchVirus-based gene therapy research