A Co-registration Pipeline for Multimodal MALDI and Confocal Imaging Analysis of Stem Cell Colonies
Arina Nikitina, Danning Huang, Li Li, Nicholas Peterman, Sarah E. Cleavenger, Facundo M. Fernández, Melissa L. Kemp
Abstract
Multimodal mass spectrometry imaging (MSI) data presents unique big data challenges in handling and analysis. Here, we present a pipeline for co-registering matrix-assisted laser desorption/ionization MSI and confocal immunofluorescence imaging data for extracting single-cell metabolite signatures. We further describe methods and introduce software for the simultaneous analysis of these concatenated data sets, which are designed to establish a connection between cell traits of interest (shape metrics, position within sample) and the cells' own metabolic signatures.
Topics & Concepts
Mass spectrometry imagingConfocalChemistryPipeline (software)MALDI imagingMatrix-assisted laser desorption/ionizationMass spectrometrySoftwareArtificial intelligenceBiological systemComputer scienceChromatographyDesorptionOpticsPhysicsOrganic chemistryBiologyAdsorptionProgramming languageMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry StudiesBacterial Identification and Susceptibility Testing