Study on detection of soybean components in edible oil with ladder-shape melting temperature isothermal amplification (LMTIA) assay
Menglin Gu, Fugang Xiao, Borui Wang, Yaoxuan Zhang, Changhe Ding, Guozhi Zhang, Deguo Wang
Abstract
O and genomic DNA (gDNA) from corn, rapeseed, cottonseed, sesame, chili, chicken, pork, beef, and mutton as negative controls to test the false positives of the LMTIA assay. The established LMTIA assay gave a sensitivity of 1 pg at an optimal temperature of 57 °C. The Edible Oil DNA Extraction Kit was suitable for the LMTIA assay to detect soybean components in refined plant oil. No false positives occurred from all negative controls. This study successfully established the LMTIA assay for the detection of soybean ITS genes in edible oils, which could be used to detect soybean components in edible oils.
Topics & Concepts
Isothermal processLoop-mediated isothermal amplificationMelting temperatureSoybean oilChemistryEdible oilChromatographyMaterials scienceAnalytical Chemistry (journal)ThermodynamicsFood scienceComposite materialPhysicsBiochemistryDNAIdentification and Quantification in FoodAdvanced Chemical Sensor TechnologiesMeat and Animal Product Quality