Multiplex detection of bacterial pathogens by PCR/SERS assay
Nana Lyu, Phani R. Potluri, Vinoth Kumar Rajendran, Yuling Wang, Anwar Sunna
Abstract
. Wherein, specific primers for amplifying each target gDNA were applied, followed by applying SERS nanotags functionalized with complementary DNA probes and Raman reporters for specific identification of the target bacterial pathogens. The PCR/SERS assay showed high specificity and sensitivity for genotyping bacterial pathogen gDNA, whereby as few as 100 copies of the target gDNA could be detected. With high sensitivity and the convenience of standard PCR amplification, the proposed assay shows great potential for the sensitive detection of multiple pathogen infections to aid clinical decision-making.
Topics & Concepts
MultiplexMultiplex polymerase chain reactionMicrobiologyMycobacterium smegmatisBacteriaBiologyChemistryPolymerase chain reactionGeneMedicineBioinformaticsBiochemistryGeneticsPathologyMycobacterium tuberculosisTuberculosisBiosensors and Analytical DetectionIdentification and Quantification in FoodBacterial Identification and Susceptibility Testing