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Reverse transcription–enzymatic recombinase amplification coupled with CRISPR-Cas12a for rapid detection and differentiation of PEDV wild-type strains and attenuated vaccine strains

Kankan Yang, Yue-qiao Liang, Yanan Li, Qi Liu, Wuyin Zhang, Dongdong Yin, Xiangjun Song, Ying Shao, Jian Tu, Kezong Qi

2021Analytical and Bioanalytical Chemistry35 citationsDOIOpen Access PDF

Topics & Concepts

Porcine epidemic diarrhea virusPorcine circovirusCRISPRVirologyBiologyTrans-activating crRNAMultiplexAmpliconRecombinase Polymerase AmplificationCoronavirusLoop-mediated isothermal amplificationMolecular biologyGeneVirusGeneticsGenome editingPolymerase chain reactionInfectious disease (medical specialty)DNAMedicineCoronavirus disease 2019 (COVID-19)DiseasePathologyAnimal Virus Infections StudiesVirus-based gene therapy researchViral gastroenteritis research and epidemiology
Reverse transcription–enzymatic recombinase amplification coupled with CRISPR-Cas12a for rapid detection and differentiation of PEDV wild-type strains and attenuated vaccine strains | Litcius