Ventral tegmental area GABA, glutamate, and glutamate‐GABA neurons are heterogeneous in their electrophysiological and pharmacological properties
Jorge Miranda‐Barrientos, Ian Chambers, Smriti Mongia, Bing Liu, Huiling Wang, Gabriel E. Mateo‐Semidey, Elyssa B. Margolis, Shiliang Zhang, Marisela Morales
Abstract
Abstract The ventral tegmental area (VTA) contains dopamine neurons intermixed with GABA‐releasing (expressing vesicular GABA transporter, VGaT), glutamate‐releasing (expressing vesicular glutamate transporter 2, VGluT2), and glutamate‐GABA co‐releasing (co‐expressing VGluT2 and VGaT) neurons. By delivering INTRSECT viral vectors into the VTA of double vglut2‐Cre/vgat‐Flp transgenic mice, we targeted specific VTA cell populations for ex vivo recordings. We found that VGluT2 + VGaT − and VGluT2 + VGaT + neurons on average had relatively hyperpolarized resting membrane potential, greater rheobase, and lower spontaneous firing frequency compared to VGluT2 − VGaT + neurons, suggesting that VTA glutamate‐releasing and glutamate‐GABA co‐releasing neurons require stronger excitatory drive to fire than GABA‐releasing neurons. In addition, we detected expression of Oprm1mRNA (encoding µ opioid receptors, MOR) in VGluT2 + VGaT − and VGluT2 − VGaT + neurons, and that the MOR agonist DAMGO hyperpolarized neurons with these phenotypes. Collectively, we demonstrate the utility of the double transgenic mouse to access VTA glutamate, glutamate‐GABA, and GABA neurons to determine their electrophysiological properties. Significant statement Some physiological properties of VTA glutamate‐releasing and glutamate‐GABA co‐releasing neurons are distinct from those of VTA GABA‐releasing neurons. µ‐opioid receptor activation hyperpolarizes some VTA glutamate‐releasing and some GABA‐releasing neurons.