High-Yielding Terpene-Based Biofuel Production in <i>Rhodobacter capsulatus</i>
Yang Zhang, Xiaohui Song, Yumeng Lai, Qiwen Mo, Jifeng Yuan
Abstract
. The above strategies enabled a 35-fold higher titer of bisabolene than that of the starting strain, reaching 1089.7 mg/L from glucose in a shake flask. The engineered strain produced 9.8 g/L bisabolene with a yield of >0.196 g/g-glucose under the two-phase fed-batch fermentation, which corresponds to >78% of theoretical maximum. Taken together, our work represents one of the pioneering studies to demonstrate PNSB as a promising platform for terpene-based advanced biofuel production.
Topics & Concepts
RhodobacterTerpeneBiofuelFermentationMetabolic engineeringHeterologousChemistryBiochemistryFood scienceBiologyBiotechnologyEnzymeMutantGenePlant biochemistry and biosynthesisMicrobial Metabolic Engineering and BioproductionEnzyme Catalysis and Immobilization