Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
Anna S. Efimova, Maria A. Ustimova, Nelly S. Chmelyuk, Maxim A. Abakumov, Yu. V. Fedorov, Оlga А. Fedorova
Abstract
New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influenced their binding modes, binding affinities, and spectroscopic responses. Biological experiments showed the low toxicity of the obtained dyes and their applicability as selective dyes for mitochondria in living cells.
Topics & Concepts
QuinolineBinding affinitiesFluorescenceAffinitiesChemistryDNAMitochondrionResidue (chemistry)PyridineCellStereochemistryBiophysicsCombinatorial chemistryBiochemistryBiologyOrganic chemistryReceptorPhysicsQuantum mechanicsMolecular Sensors and Ion DetectionDNA and Nucleic Acid ChemistryLuminescence and Fluorescent Materials