High sensitive Ratiometric fluorescent Aptasensor with AIE properties for Deoxynivalenol (DON) detection
Yuandong Lin, Nian Chun Lü, Ji Ma, Jun‐Hu Cheng, Da‐Wen Sun
Abstract
An emerging fluorescent ratiometric aptasensor based on gold nanoclusters (AuNCs) with aggregation-induced emission (AIE) properties was prepared and studied for deoxynivalenol (DON) detection. The ratiometric aptasensor used red fluorescent AuNCs 620 labelled with DON aptamer (Apt-AuNCs 620 ) as an indicator and green fluorescent AuNCs 519 modified by complementary DNA (cDNA) and magnetic beads (MBs) as internal reference, namely MBs-cDNA-AuNCs 519 . Under the optimal conditions, the aptasensor exhibited two good linear ranges of 0.1–50 and 50–5000 pg/mL for DON detection with coefficient of determination (R 2 ) of 0.9937 and 0.9928, respectively, and the low detection limit (LOD) of 4.09 pg/mL was achieved. Furthermore, this aptasensor was feasible to detect DON in positive wheat samples, and the results were in line with those from HPLC and ELISA, thus providing a promising route to detect DON with high sensitivity in cereals, even for other mycotoxins by replacing the suitable aptamer and cDNA. • Two AuNCs with AIE properties were prepared to construct a ratiometric aptasensor. • DON could achieve highly specific detection based on the proposed aptasensor. • A relatively low limit of detection of 4.09 pg/mL was achieved. • The applicability of the aptasensor in wheat samples was validated.