A Cytopathic Effect-Based Tissue Culture Method for HCoV-OC43 Titration Using TMPRSS2-Expressing VeroE6 Cells
Ryohei Hirose, Naoto Watanabe, Risa Bandou, Takuma Yoshida, Tomo Daidoji, Yuji Naito, Yoshito Itoh, Takaaki Nakaya
Abstract
assay using CPE evaluation with TMPRSS2-expressing VeroE6/TMPRSS2 cells that provides the same accuracy as the conventional IPA-based viral titration and does not require any staining procedures using antibodies or substrates. This titration method will greatly contribute to future research on HCoV-OC43 by allowing simple, low-cost, and accurate titration of this virus.
Topics & Concepts
Cytopathic effectTMPRSS2VirologyCell cultureSerine proteaseBiologyCellImmunoperoxidaseVirusMolecular biologyMicrobiologyProteaseImmunologyMedicineCoronavirus disease 2019 (COVID-19)PathologyGeneticsAntibodyEnzymeBiochemistryInfectious disease (medical specialty)DiseaseMonoclonal antibodySARS-CoV-2 and COVID-19 ResearchCOVID-19 Clinical Research StudiesSARS-CoV-2 detection and testing