<i>Vitis vinifera</i>leaf extract liposomal Carbopol gel preparation's potential wound healing and antibacterial benefits:<i>in vivo</i>, phytochemical, and computational investigation
Abeer H. Elmaidomy, Soad A. Mohamad, Mahmoud Abdelnaser, Ramadan Yahia, Fatma Alzahraa Mokhtar, Faisal Alsenani, Moutaz Y. Badr, Safa Almaghrabi, Faisal H. Altemani, Mubarak A. Alzubaidi, Entesar Ali Saber, Mahmoud A. Elrehany, Usama Ramadan Abdelmohsen, Ahmed M. Sayed
Abstract
evaluation proved the ability of VVL-liposomal gel to gradually release the drugs in a sustained manner with greater complete wound healing effect and tissue repair after 7 days of administration, with a significant decrease in bacterial count compared with the crude extract. Phytochemical investigation of the crude extract of the leaves yielded fourteen compounds: two new stilbenes (1, 2), along with twelve known ones (3-14). Furthermore, a computational study was conducted to identify the genes and possible pathways responsible for the anti-MRSA activity of the isolated compounds, and inverse docking was used to identify the most likely molecular targets that could mediate the extract's antibacterial activity. Gyr-B was discovered to be the best target for compounds 1 and 2. Hence, VVL-liposomal gel can be used as a novel anti-dermatophytic agent with potent wound healing and anti-MRSA capacity, paving the way for future clinical research.