Blueberry Anthocyanins Extract Attenuates Acrylamide‐Induced Oxidative Stress and Neuroinflammation in Rats
Zizhuang Fang, Yinghua Luo, Chen Ma, Dong Li, Fang Chen
Abstract
Acrylamide (AA) is a widespread environmental and dietary‐derived neurotoxin, which can induce oxidative stress and associated inflammation in the brain. Anthocyanins widely occur as natural antioxidant and anti‐inflammatory phytochemicals. Herein, the protective effects of blueberry anthocyanins extract (BAE) against AA‐induced neurotoxicity were investigated in rats. The rats were pretreated with BAE (175 mg/kg body weight/day) by oral gavage for the first 7 days, followed by the co‐administration of BAE and AA (35 mg/kg body weight/day) by oral gavage for the next 12 days. Results showed that BAE significantly decreased the malondialdehyde (MDA) production, and increased glutathione (GSH) and antioxidant enzyme levels; and it also suppressed microglial activation, astrocytic reaction, and pro‐inflammatory cytokine expressions. Furthermore, BAE elevated the extracellular signal‐related kinase (ERK)/cAMP response elements binding protein (CREB)/brain‐derived neurotrophic factor (BDNF) pathway, and relieved the accumulation of amyloid beta (A β ) 1‐42 and 1‐40 after AA exposure. Consequently, AA‐induced neuronal necrosis and downregulation of synaptosomal‐associated protein 25 (SNAP‐25) were attenuated by BAE in the hippocampus and cerebral cortex. In conclusion, BAE can exert a protective function on neurons and synapses against AA‐induced oxidative stress and neuroinflammation.