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Use of Plasma Metagenomic Next-generation Sequencing for Pathogen Identification in Pediatric Endocarditis

Rachel K. To, Nanda Ramchandar, Aamisha Gupta, Alice Pong, Christopher Cannavino, Jennifer Foley, Lauge Farnaes, Nicole G. Coufal

2020The Pediatric Infectious Disease Journal39 citationsDOI

Abstract

Pediatric infective endocarditis incurs significant morbidity and generally occurs among children with underlying heart disease. Identification of a pathogen is critical in determining appropriate therapy. However, standard diagnostic testing has limited sensitivity. We describe a case series of children with infective endocarditis in whom plasma next-generation sequencing (Karius, Redwood, CA) identified an organism in 8 of 10 cases.

Topics & Concepts

Infective endocarditisPathogenEndocarditisMetagenomicsIdentification (biology)DNA sequencingMedicineDiseaseIntensive care medicineBiologyComputational biologyImmunologyInternal medicineGeneticsGeneEcologyInfective Endocarditis Diagnosis and ManagementAntimicrobial Resistance in StaphylococcusStreptococcal Infections and Treatments
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