Analytical methods for detection of human cytomegalovirus clinched biosensor a cutting-edge diagnostic tool
Mohd. Rahil Hasan, Pradakshina Sharma, Nigar Anzar, C.S. Pundir, Roberto Pilloton, Jagriti Narang, Nagaraj P. Shetti
Abstract
Human cytomegalovirus (HCMV) is a beta herpes-virus, which affects human being as a lifelong infection. HCMV is the prominent cause for the infections of congenital with a 1.0–2.4% incidence of live-births, along with possible severe classic cytomegalovirus. Crucial HCMV infection is usually asymptomatic in healthy hosts but it can cause severe or sometimes fatal illness in immuno-compromised neonates and individuals. Various conventional methods such as PCR, virus isolation, antigenemia test, histological and serological are available for detection of HCMV. Among all the analytical techniques, biosensors clinched as the most advanced technology, which offers many features such as simplicity, inexpensive, highly sensitive, and effective approach. The future of diagnosis will rely on the development of point-of-care devices, which can be used at the site of need, resource-restricted settings, and provides affordability. This review describes various analytical methods for the detection of HCMV emphasizing biosensing methods.