Adaptive peptide dispersions enable drying-induced biomolecule encapsulation
Dhwanit Dave, Salma Kassem, Maëva Coste, Lele Xu, Mona Tayarani-Najjaran, Darjan Podbevšek, Pablo de León, Sheng Zhang, Luis E. Ortuno Macias, Deborah Sementa, María Pérez-Ferreiro, Nooshin Sadat Ayati, Muniyat A. Choudhury, Kelly Veerasammy, Selma Doganata, Tiffany Zhong, Cory Weng, Jorge Morales, Denize C. Favaro, Mateusz Marianski, So Yeon Ahn, Allie C. Obermeyer, Tong Wang, Tai‐De Li, Xi Chen, Raymond S. Tu, Ye He, Rein V. Ulijn
Abstract
Peptides are promising building blocks of designer materials with wide-ranging applications. These materials are stabilized by directional hydrogen-bonding patterns, giving rise to one-dimensional or two-dimensional assembly. It remains a challenge to mimic biology’s context-adaptive and flexible structures. Here we introduce minimalistic tripeptide sequences that form highly soluble dynamic ensembles through multivalent side-chain interactions. We observe these supramolecular dispersions undergo drying-induced sequential liquid–liquid phase separation followed by solidification, resulting in the formation of films of stiff, densely packed and porous peptide microparticles that can be instantaneously redispersed upon the re-introduction of water. Air-drying of peptide dispersions in the presence of proteins or small-molecule payloads results in efficient encapsulation and the retention of protein stability after redispersion, showing promise for the emulsification, encapsulation, protection and storage of biomacromolecules. The mechanism resembles the protective strategies in natural systems during desiccation, which rely on liquid–liquid phase separation to survive extreme conditions. Here the authors design tripeptides that form dynamic soluble dispersions and undergo phase separation upon drying to assemble into porous particles. This evaporation-driven emulsification can be harnessed to encapsulate and stabilize biomolecules.