Interaction of macrocyclic gadolinium-based MR contrast agents with Type I collagen. Equilibrium and kinetic studies
N. Guidolin, Fabio Travagin, Giovanni B. Giovenzana, Adrienn Vágner, Sophia Lotti, Federica Chianale, Ernő Brücher, Federico Maisano, Miles A. Kirchin, Fabio Tedoldi, Alice Giorgini, Sonia Colombo Serra, Zsolt Baranyai
Abstract
The interactions of gadoterate meglumine, gadobutrol, gadoteridol and Gd(HB-DO3A) with bovine Type I collagen were investigated by ultrafiltration and dialysis. The affinity of the four agents to collagen is similar. However, the maximum adsorbed amount of GdIII-complexes decreases in the following order: gadoterate meglumine > gadobutrol > gadoteridol > Gd(HB-DO3A). Calculations with the open three-compartment model reveal that the structural homologs gadoteridol and Gd(HB-DO3A) have a lower adsorption onto collagen, which may explain the less prolonged in vivo retention of gadoteridol observed in soft tissues of rats.