Identification and Differentiation of Pseudomonas Species in Field Samples Using an <i>rpoD</i> Amplicon Sequencing Methodology
Jonas Greve Lauritsen, Morten Lindqvist Hansen, Pernille Kjersgaard Bech, Lars Jelsbak, Lone Gram, Mikael Lenz Strube
Abstract
A high-throughput sequencing-based method for profiling of Pseudomonas species in soil microbiomes was developed and identified more species than 16S rRNA gene sequencing or cultivation. Pseudomonas species are used as biocontrol organisms and plant growth-promoting agents, and the method will allow tracing of specific species of Pseudomonas as well as enable screening of environmental samples for further isolation and exploitation.
Topics & Concepts
BiologyPseudomonasAmplicon16S ribosomal RNARibosomal RNAGeneticsMicrobiologyPolymerase chain reactionGeneBacteriaGenomics and Phylogenetic StudiesMicrobial Community Ecology and PhysiologyPlant-Microbe Interactions and Immunity