Itaconate is a covalent inhibitor of the <i>Mycobacterium tuberculosis</i> isocitrate lyase
Brooke X. C. Kwai, Annabelle J. Collins, Martin Middleditch, Jonathan Sperry, Ghader Bashiri, Ivanhoe K. H. Leung
Abstract
. Herein, we report that ICLs form a covalent adduct with itaconate through their catalytic cysteine residue. These results reveal atomic details of itaconate inhibition and provide insights into the catalytic mechanism of ICLs.
Topics & Concepts
Isocitrate lyaseMycobacterium tuberculosisCovalent bondChemistryMicrobiologyTuberculosisBiochemistryBiologyEnzymeOrganic chemistryMedicineGlyoxylate cyclePathologyTuberculosis Research and EpidemiologyMycobacterium research and diagnosisBiochemical and Molecular Research