MicroRNA-21 expression in single living cells revealed by fluorescence and SERS dual-response microfluidic droplet platform
Dan Sun, Fanghao Cao, Xuan Yi, Hongyan Zhu, Guohua Qi, Weiqing Xu, Shuping Xu
Abstract
, nondestructive determination of miR-21 secreted by single cells, avoiding cell lysis and tedious time-consuming steps of miR-21 isolation. As a result, the miR-21 expressed by various types of single cells was investigated by fluorescence imaging and the cellular heterogeneity in miR-21 expression was evaluated accurately and quantitatively by SERS. This research would provide important reference information for understanding the effects of miRNAs on cancer diseases at the single-cell level.
Topics & Concepts
MicrofluidicsNanoprobeFluorescenceSingle-cell analysismicroRNANanotechnologyLysisIn situCellChemistryBiophysicsFluorescence-lifetime imaging microscopyNanoparticleMaterials scienceBiologyBiochemistryGenePhysicsQuantum mechanicsOrganic chemistryAdvanced biosensing and bioanalysis techniquesExtracellular vesicles in diseaseRNA Interference and Gene Delivery