Using Collision Cross Section Distributions to Assess the Distribution of Collision Cross Section Values
Aidan P. France, Lukasz G. Migas, Eleanor Sinclair, Bruno Bellina, Perdita E. Barran
Abstract
CCS for native-like proteins which are largely similar to those obtained on DTIMS instruments. We collate previously reported values of CCS for these proteins in the form of histograms which bear a remarkable similarity to the CCS distributions, reflecting the conformational heterogeneity of proteins and also how conformer populations can be altered on transfer from solution to the detector. This gives concern for some caution when calibrating sample protein drift times simply with single numeric CCS values.
Topics & Concepts
Ion-mobility spectrometryChemistryCollisionIonCross section (physics)Gas phaseComparabilityCalibrationMass spectrometryPhase (matter)Analytical Chemistry (journal)Computational physicsStatisticsChromatographyPhysicsPhysical chemistryComputer scienceMathematicsComputer securityCombinatoricsQuantum mechanicsOrganic chemistryMass Spectrometry Techniques and ApplicationsAnalytical Chemistry and ChromatographyAdvanced Proteomics Techniques and Applications