Tracking trash to treasure: <i>in situ</i> monitoring of single microbial cell oil biosynthesis from waste cooking oil using Raman spectroscopy and imaging
Jiro Karlo, Víctor Carrasco-Navarro, Arto Koistinen, Surya Pratap Singh
Abstract
, an oleaginous yeast grown in hydrophilic (glucose) as well as hydrophobic carbon sources (cooking oil and waste cooking oil). This study also combines the RrSIP approach with Raman imaging for temporal visualization of the distribution and turnover dynamics of the SMCO pool in a single cell. Our finding provides a unique perspective utilizing optical spectroscopy methods for lysis-free SMCO analysis and holds potential for prospective utility as an adjunct tool in bioprocess and biofuel industries.
Topics & Concepts
Raman spectroscopyChemistryYeastIn situOrganic chemistryBiochemistryPhysicsOpticsMicrobial Metabolic Engineering and BioproductionEnzyme Catalysis and ImmobilizationMetabolomics and Mass Spectrometry Studies