Measuring anion transport selectivity: a cautionary tale
Xin Wu, Philip A. Gale
Abstract
pH-dependent liposomal assays are often used to determine anion selectivity in transmembrane anion transport experiments. We discuss the validity and limitations of these assays, and provide guidelines for their use to avoid misleading results.
Topics & Concepts
SelectivityChemistryCombinatorial chemistryIonIon transporterBiophysicsBiochemistryBiologyOrganic chemistryCatalysisElectrochemical Analysis and ApplicationsMolecular Sensors and Ion DetectionDrug Transport and Resistance Mechanisms