Construction of flexible paper-based sensor for label-free recognition of histamine in cow meat samples by conductive nano-silver ink: a new platform for the analysis of biogenic amines towards early diagnosis of meat spoil
Rokhsareh Ebrahimi, Mohammad Hasanzadeh, Nasrin Shadjou
Abstract
a pen-on-paper technique. The fabrication process of the microsensor was thoroughly characterized through methods like field emission scanning electron microscope (FE-SEM), Energy Dispersive X-ray (EDAX), and Atomic Force microscopy (AFM). Key findings indicate that the microsensor successfully detects histamine concentrations across a broad dynamic range of 10 to 1000 nM, with a lower limit of quantification set at 10 nM. Validation of the sensor's performance was conducted using electrochemical tools such as cyclic voltammetry and square wave voltammetry, confirming its efficacy for real-time histamine monitoring in food products and biological environments. Additionally, the study underscores the sensor's excellent selectivity, long-term stability, and lightning-fast responsiveness, positioning it as a highly promising tool to enhance food safety and quality assurance.