Green hydrothermal synthesis of multifunctional carbon dots from cassava pulps for metal sensing, antioxidant, and mercury detoxification in plants
Teera Watcharamongkol, Pacharaphon Khaopueak, Chuleekorn Seesuea, Kanokorn Wechakorn
Abstract
Carbon dots (CDs) have been attracted to nanocarbon materials for metal ion sensing, biological activity, and plant phytotoxicity due to their excellent photophysical properties, such as low cytotoxicity, high quantum yield, tunable fluorescence emission, and biocompatibility. Cassava pulp, which consists mainly of starch, has been identified as a low-cost biomass waste from the cassava starch industry. Therefore, this research developed CDs and nitrogen-doped CDs (NCDs) from cassava pulp using a one-step hydrothermal process in deionized water at 200°C. The effects of the synthesis conditions, including reaction time (6-24 hours) and the nitrogen doping derivatives, were also investigated. CDs and ethylenediamine doped-NCDs exhibited tunable fluorescence emission, strong quantum yield, high photostability, and tolerance to photobleaching. Furthermore, the potential applications of CDs-12h were demonstrated such as fluorescent sensor for metal ion sensing, antioxidant activity, and mercury detoxification in plants. Fluorescence quenching of the CDs-12h via both static and dynamic quenching mechanisms was observed in the presence of several metal ions such as Hg2+, Cu2+, and Fe3+ with the detection limit in micromolar levels and further applied to real water samples with good recovery and acceptable relative standard derivation. The paper test strip coated with CDs-12h could also detect these metal ions under UV light. CDs and NCDs-EDA also showed potential DPPH radical scavenging activity and alleviated mercury toxicity in the Chinese cabbage seedlings with the incubation of CDs-12h and NCDs-EDA-12h (30 mg/L).