Accelerated Multiphosphorylated Peptide Synthesis
Dana Grunhaus, Estefanía Rossich Molina, Roni Cohen, Tamar Stein, Assaf Friedler, Mattan Hurevich
Abstract
Preparing phosphorylated peptides with multiple adjacent phosphorylations is synthetically difficult, leads to β-elimination, results in low yields, and is extremely slow. We combined synthetic chemical methodologies with computational studies and engineering approaches to develop a strategy that takes advantage of fast stirring, high temperature, and a very low concentration of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) to produce multiphosphorylated peptides at an extremely rapid time and high purity.
Topics & Concepts
ChemistryCombinatorial chemistryPeptidePeptide synthesisNanotechnologyMaterials scienceBiochemistryChemical Synthesis and AnalysisRNA and protein synthesis mechanismsPeptidase Inhibition and Analysis