Precise diagnosis of tumor cells and hemocytes using ultrasensitive, stable, selective cuprous oxide composite SERS bioprobes assisted with high-efficiency separation microfluidic chips
Yujiao Xie, Lei Xu, J.Y. Zhang, Chenguang Zhang, Yue Hu, Zhouxu Zhang, Guoxin Chen, Shuyan Qi, Xiawei Xu, Jing Wang, Wenzhi Ren, Jie Lin, Aiguo Wu
Abstract
O@Ag composites significantly improved electromagnetic enhancement and charge transfer effects at 532 nm, providing a reliable basis for the label-free SERS detection. After the cancer cells in blood were separated by a spiral inertial microfluidic chip (purity >80%), machine learning-assisted linear discriminant analysis (LDA) successfully distinguished three types of cancer cells and WBCs with high accuracy (>90%). In conclusion, this study provides a profound reference for the rational design of SERS probes and the efficient diagnosis of malignant tumors.
Topics & Concepts
MicrofluidicsMaterials scienceSubstrate (aquarium)Microfluidic chipNanotechnologyCirculating tumor cellComposite numberCancer cellLab-on-a-chipCancerBiologyComposite materialMetastasisGeneticsEcologyGold and Silver Nanoparticles Synthesis and ApplicationsBiosensors and Analytical DetectionSpectroscopy Techniques in Biomedical and Chemical Research