Single-cell architecture and functional requirement of alternative splicing during hematopoietic stem cell formation
Fang Wang, Puwen Tan, Pengcheng Zhang, Yue Ren, Jie Zhou, Yunqiao Li, Siyuan Hou, Shuaili Li, Linlin Zhang, Yanni Ma, Chaojie Wang, Wanbo Tang, Xiaoshuang Wang, Yue Huo, Yongfei Hu, Tianyu Cui, Chao Niu, Dong Wang, Bing Liu, Yu Lan, Jia Yu
Abstract
deficiency from endothelial cells affected the splicing pattern of several master hematopoietic regulators and significantly impaired HSC generation. These results redefine our understanding of the dynamic HSC developmental transcriptome and demonstrate that elaborately controlled RNA splicing governs cell fate in HSC formation.
Topics & Concepts
BiologyStem cellRNA splicingCell biologyTranscriptomeHaematopoiesisHematopoietic stem cellAlternative splicingGene expression profilingRNA-binding proteinEmbryonic stem cellComputational biologyRNAGeneticsGene isoformGene expressionGeneSingle-cell and spatial transcriptomicsRNA Research and SplicingRNA modifications and cancer