The differential effects of endogenous cathepsin and microorganisms on changes in the texture and flavor substances of grouper (<i>Epinephelus coioides</i>) fillets
Xicai Zhang, Jing Xie
Abstract
on the 18th day, and the activity of protease was not significantly affected. On the 6th day, the hardness of the iodoacetic acid treatment group decreased by 8%, while the ProClin 300 treatment group decreased by 28%, and changes in the free amino acids and volatile substances significantly exceeded those of the iodoacetic acid treatment group, indicating that endogenous protease was the main factor in the texture deterioration. A first-order exponential decay model indicated that cathepsin L was the most important protease for reducing the hardness of grouper fillets, and changes in the content of free amino acids and volatile substances indicated that microorganisms played a more important role in the deterioration of flavor substances compared to that played by endogenous protease.