<scp>STAT3</scp>modulates<scp>CD4</scp><sup>+</sup>T mitochondrial dynamics and function in aging
Emelia Zukowski, Marco Sannella, Jack Donato Rockhold, Gabriella H. Kalantar, Jingting Yu, Sara Santa-Cruz Calvo, Madison K. Kuhn, Nasun Hah, Ling Ouyang, Tzu‐Wen Wang, Lyanne Murphy, Heather Marszalkowski, Kaleigh Gibney, Micah J. Drummond, Elizabeth A. Proctor, Hatice Hastürk, Barbara S. Nikolajczyk, Leena P. Bharath
Abstract
Aging promotes numerous intracellular changes in T cells that impact their effector function. Our data show that aging promotes an increase in the localization of STAT3 to the mitochondria (mitoSTAT3), which promotes changes in mitochondrial dynamics and function and T-cell cytokine production. Mechanistically, mitoSTAT3 increased the activity of aging T-cell mitochondria by increasing complex II. Limiting mitoSTAT3 using a mitochondria-targeted STAT3 inhibitor, Mtcur-1 lowered complex II activity, prevented age-induced changes in mitochondrial dynamics and function, and reduced Th17 inflammation. Exogenous expression of a constitutively phosphorylated form of STAT3 in T cells from young adults mimicked changes in mitochondrial dynamics and function in T cells from older adults and partially recapitulated aging-related cytokine profiles. Our data show the mechanistic link among mitoSTAT3, mitochondrial dynamics, function, and T-cell cytokine production.